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ACS Synth Biol. 2017 Sep 15;6(9):1710-1721. doi: 10.1021/acssynbio.7b00059. Epub 2017 May 26.

A Fluorescent Split Aptamer for Visualizing RNA-RNA Assembly In Vivo.

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Department of Biochemistry, University of Missouri , Columbia, Missouri 65211, United States.
Bond Life Sciences Center, University of Missouri , Columbia, Missouri 65211, United States.
Department of Molecular Microbiology and Immunology, University of Missouri , Columbia, Missouri 65212, United States.
Department of Bioengineering, University of Missouri , Columbia, Missouri 65211, United States.


RNA-RNA assembly governs key biological processes and is a powerful tool for engineering synthetic genetic circuits. Characterizing RNA assembly in living cells often involves monitoring fluorescent reporter proteins, which are at best indirect measures of underlying RNA-RNA hybridization events and are subject to additional temporal and load constraints associated with translation and activation of reporter proteins. In contrast, RNA aptamers that sequester small molecule dyes and activate their fluorescence are increasingly utilized in genetically encoded strategies to report on RNA-level events. Split-aptamer systems have been rationally designed to generate signal upon hybridization of two or more discrete RNA transcripts, but none directly function when expressed in vivo. We reasoned that the improved physiological properties of the Broccoli aptamer enable construction of a split-aptamer system that could function in living cells. Here we present the Split-Broccoli system, in which self-assembly is nucleated by a thermostable, three-way junction RNA architecture and fluorescence activation requires both strands. Functional assembly of the system approximately follows second-order kinetics in vitro and improves when cotranscribed, rather than when assembled from purified components. Split-Broccoli fluorescence is digital in vivo and retains functional modularity when fused to RNAs that regulate circuit function through RNA-RNA hybridization, as demonstrated with an RNA Toehold switch. Split-Broccoli represents the first functional split-aptamer system to operate in vivo. It offers a genetically encoded and nondestructive platform to monitor and exploit RNA-RNA hybridization, whether as an all-RNA, stand-alone AND gate or as a tool for monitoring assembly of RNA-RNA hybrids.


RNA AND gate; RNA interaction; RNA synthetic biology; binary aptamer; cotranscriptional RNA assembly; fluorescence complementation

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