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ACS Appl Mater Interfaces. 2017 Jun 7;9(22):18434-18439. doi: 10.1021/acsami.7b04784. Epub 2017 May 30.

Preservation of DNA Nanostructure Carriers: Effects of Freeze-Thawing and Ionic Strength during Lyophilization and Storage.

Zhu B1,2, Zhao Y1,2, Dai J1,2, Wang J1,2, Xing S1, Guo L1, Chen N1, Qu X3, Li L3, Shen J3, Shi J4, Li J1, Wang L1.

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Division of Physical Biology and Bioimaging Center, Shanghai Synchrotron Radiation Facility; CAS Key Laboratory of Interfacial Physics and Technology; Shanghai Institute of Applied Physics, Chinese Academy of Sciences , Shanghai 201800, China.
University of Chinese Academy of Sciences , Beijing 10049, China.
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University , Shanghai 200241, China.
UCB Pharma , 208 Bath Road, Slough SL1 3WE, United Kingdom.


DNA nanostructures have attracted wide interest in biomedical applications, especially as nanocarriers for drug delivery. Therefore, it is important to ensure the structural integrity of DNA nanostructures under ambient temperature storage. In this study, we examined lyophilization-based preservation of DNA nanostructures by investigating the structural integrity of different DNA nanostructures reconstituted from lyophilization. We demonstrated that lyophilization under appropriate ionic strength is amenable to the storage of DNA nanostructures. Compared with that stored in liquid solution, DNA nanostructure carriers reconstituted from lyophilization showed significantly better structural integrity after an accelerated aging test equivalent to 100-day room-temperature storage.


DNA nanostructures; ionic strength; lyophilization; storage; structure friendly

[Indexed for MEDLINE]

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