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J Cell Sci. 2017 Jul 15;130(14):2266-2276. doi: 10.1242/jcs.203000. Epub 2017 May 25.

Mechanisms mitigating problems associated with multiple kinetochores on one microtubule in early mitosis.

Author information

1
Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
2
Data Analysis Group, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK.
3
Centre for Gene Regulation and Expression, School of Life Sciences, University of Dundee, Dundee DD1 5EH, UK t.tanaka@dundee.ac.uk.

Abstract

Proper chromosome segregation in mitosis relies on correct kinetochore interaction with spindle microtubules. In early mitosis, each kinetochore usually interacts with the lateral side of each microtubule and is subsequently tethered at the microtubule end. However, since eukaryotic cells carry multiple chromosomes, multiple kinetochores could occasionally interact with a single microtubule. The consequence of this is unknown. Here, we find that, although two kinetochores (two pairs of sister kinetochores) can interact with the lateral side of one microtubule, only one kinetochore can form a sustained attachment to the microtubule end in budding yeast (Saccharomyces cerevisiae). This leads to detachment of the other kinetochore from the microtubule end (or a location in its proximity). Intriguingly, in this context, kinetochore sliding along a microtubule towards a spindle pole delays and diminishes discernible kinetochore detachment. This effect expedites collection of the entire set of kinetochores to a spindle pole. We propose that cells are equipped with the kinetochore-sliding mechanism to mitigate problems associated with multiple kinetochores on one microtubule in early mitosis.

KEYWORDS:

Budding yeast; Early mitosis; End-on attachment; Kinetochore; Kinetochore sliding; Microtubule

PMID:
28546446
PMCID:
PMC5536920
DOI:
10.1242/jcs.203000
[Indexed for MEDLINE]
Free PMC Article

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