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Biochem Biophys Res Commun. 2017 Jul 22;489(2):142-148. doi: 10.1016/j.bbrc.2017.05.114. Epub 2017 May 22.

Catechins activate muscle stem cells by Myf5 induction and stimulate muscle regeneration.

Author information

1
Department of Life Sciences, School of Life Sciences and Biotechnology, Korea University, Seoul 02841, South Korea.
2
College of Pharmacy, Ewha Woman's University, Seoul 03760, South Korea.
3
Department of Life Sciences, School of Life Sciences and Biotechnology, Korea University, Seoul 02841, South Korea. Electronic address: jh_hong@korea.ac.kr.

Abstract

Muscle weakness is one of the most common symptoms in aged individuals and increases risk of mortality. Thus, maintenance of muscle mass is important for inhibiting aging. In this study, we investigated the effect of catechins, polyphenol compounds in green tea, on muscle regeneration. We found that (-)-epicatechin gallate (ECG) and (-)-epigallocatechin-3-gallate (EGCG) activate satellite cells by induction of Myf5 transcription factors. For satellite cell activation, Akt kinase was significantly induced after ECG treatment and ECG-induced satellite cell activation was blocked in the presence of Akt inhibitor. ECG also promotes myogenic differentiation through the induction of myogenic markers, including Myogenin and Muscle creatine kinase (MCK), in satellite and C2C12 myoblast cells. Finally, EGCG administration to mice significantly increased muscle fiber size for regeneration. Taken together, the results suggest that catechins stimulate muscle stem cell activation and differentiation for muscle regeneration.

KEYWORDS:

Catechins; Muscle regeneration; Myogenic differentiation; Phosphoinositide 3-kinases/Akt; Satellite cells

PMID:
28546002
DOI:
10.1016/j.bbrc.2017.05.114
[Indexed for MEDLINE]

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