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Phytomedicine. 2017 Jul 1;30:42-49. doi: 10.1016/j.phymed.2017.01.015. Epub 2017 Feb 3.

Phenethyl isothiocyanate suppresses cancer stem cell properties in vitro and in a xenograft model.

Author information

1
Natural Products Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Korea; Convergence Research Center for Smart Farm Solution, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Korea; Department of Life Science, Sogang University, Seoul 04107, Korea.
2
Natural Products Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Korea; Department of Biological Chemistry, Korea University of Science and Technology (UST), Daejeon 34113, Korea.
3
Natural Products Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Korea; Convergence Research Center for Smart Farm Solution, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Korea.
4
Convergence Research Center for Smart Farm Solution, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Korea.
5
Natural Products Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Korea; Convergence Research Center for Smart Farm Solution, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Korea; Department of Biological Chemistry, Korea University of Science and Technology (UST), Daejeon 34113, Korea.
6
Department of Life Science, Sogang University, Seoul 04107, Korea.
7
Natural Products Research Center, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Korea.
8
Convergence Research Center for Smart Farm Solution, Korea Institute of Science and Technology (KIST), Gangneung Institute of Natural Products, Gangneung, Gangwon-do 25451, Korea. Electronic address: cwnho@kist.re.kr.

Abstract

BACKGROUND:

Cancer stem cells (CSCs) are a subset of cells within the bulk of a tumor that have the ability to self-renew and differentiate, and are thus associated with cancer invasion, metastasis, and recurrence. Phenethyl isothiocyanate (PEITC) is a natural compound found in cruciferous vegetables such as broccoli and is used as a cancer chemopreventive agent; however, its effects on CSCs are little known.

PURPOSE:

To evaluate the effect of PEITC on CSCs in this study by examining CSC properties.

METHODS:

NCCIT human embryonic carcinoma cells were treated with PEITC, and the expression of pluripotency factors Oct4, Sox-2, and Nanog were evaluated by luciferase assay and western blot. Effect of PEITC on self-renewal capacity and clonogenicity were assessed with the sphere formation, soft agar assay, and clonogenic assay in an epithelial cell adhesion molecule (EpCAM)-expressing CSC model derived from HCT116 colon cancer cells using a cell sorting system. The effect of PEITC was also investigated in a mouse xenograft model obtained by injecting nude mice with EpCAM-expressing cells.

RESULTS:

We found that PEITC treatment suppressed expression of the all three pluripotency factors in the NCCIT cells, in which pluripotency factors are highly expressed. Moreover, PEITC suppressed the self-renewal capacity and clonogenicity in the EpCAM-expressing CSC model. EpCAM was used as a specific CSC marker in this study. Importantly, PEITC markedly suppressed both tumor growth and expression of three pluripotency factors in a mouse xenograft model.

CONCLUSION:

These results demonstrate that PEITC might be able to slow down or prevent cancer recurrence by suppressing CSC stemness.

KEYWORDS:

Cancer stem cells; EpCAM; Oct4; PEITC; Xenograft model

PMID:
28545668
DOI:
10.1016/j.phymed.2017.01.015
[Indexed for MEDLINE]

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