Format

Send to

Choose Destination
Mol Nutr Food Res. 2017 Oct;61(10). doi: 10.1002/mnfr.201700142. Epub 2017 Jul 20.

Within-person reproducibility and sensitivity to dietary change of C15:0 and C17:0 levels in dried blood spots: Data from the European Food4Me Study.

Author information

1
Human Nutrition Research Centre and Institute of Health and Society, Newcastle University, Newcastle upon Tyne, UK.
2
Human Nutrition Research Centre, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, UK.
3
UCD School of Agriculture and FoodScience, Institute of Food and Health, University College Dublin (UCD), Belfield, Dublin, Ireland.
4
Hugh Sinclair Unit of Human Nutrition and Institute for Cardiovascular and Metabolic Health, University of Reading, Reading, UK.
5
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre, Maastricht, The Netherlands.
6
Department of Nutrition, Food Science and Physiology, University of Navarra, Pamplona, Spain.
7
CIBER Fisiopatología de la Obesidad y Nutrición (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.
8
ZIEL Research Center of Nutrition and Food Sciences, Biochemistry Unit, Technische Universität München, Munich, Germany.
9
Department of Nutrition and Dietetics, Harokopio University, Athens, Greece.
10
National Food & Nutrition Institute, Warsaw, Poland.
11
Department of Human Nutrition, Faculty of Health Science, Medical University of Warsaw, Poland.
12
Vitas AS, Oslo, Norway.
13
Department of Nutrition, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Oslo, Norway.
14
IMDEA Alimentación, Madrid, Spain.

Abstract

SCOPE:

Previous work highlighted the potential of odd-chain length saturated fatty acids as potential markers of dairy intake. The aim of this study was to assess the reproducibility of these biomarkers and their sensitivity to changes in dairy intake.

METHODS AND RESULTS:

Fatty acid profiles and dietary intakes from food frequency questionnaires (FFQs) were measured three times over six months in the Food4Me Study. Reproducibility was explored through intra-class correlation coefficients (ICCs) and within-subject coefficients of variation (WCV). Sensitivity to changes in diet was examined using regression analysis. C15:0 blood levels showed high correlation over time (ICC: 0.62, 95% CI: 0.57, 0.68), however, the ICC for C17:0 was much lower (ICC: 0.32, 95% CI: 0.28, 0.46). The WCV for C15:0 was 16.6% and that for C17:0 was 14.6%. There were significant associations between changes in intakes of total dairy, high-fat dairy, cheese and butter and C15:0; and change in intakes of high-fat dairy and cream and C17:0.

CONCLUSION:

Results provide evidence of reproducibility of C15:0 levels over time and sensitivity to change in intake of high-fat dairy products with results comparable to the well-established biomarker of fish intake (EPA+DHA).

KEYWORDS:

Biomarkers; Dairy intake; Dried blood spots; Heptadecanoic acid; Intra-class correlation; Pentadecanoic acid

PMID:
28544702
DOI:
10.1002/mnfr.201700142
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center