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Biomed Chromatogr. 2017 Dec;31(12). doi: 10.1002/bmc.4012. Epub 2017 Jun 20.

Relationship between the UPLC-Q-TOF-MS fingerprinted constituents from Daphne genkwa and their anti-inflammatory, anti-oxidant activities.

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State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.
School of Pharmacy, Queen's University of Belfast, Belfast, Northern Ireland, UK.
Jiangsu Kanion Pharmaceutical Co. Ltd, Lianyungang, China.
Tang Center of Herbal Medicine Research and Department of Anesthesia and Critical Care, University of Chicago, Chicago, Illinois, USA.


Daphne genkwa Zucc. is a well-known medicinal plant. This study was designed to apply the ultra-high performance liquid chromatography system to establish a quality control method for D. genkwa. Data revealed that there were 15 common peaks in 10 batches of D. genkwa Sieb. Et Zucc. (Thymelaeaceae) from different provinces of China. On this basis, the fingerprint chromatogram was established to provide references for quality control. Afterwards, the chemical constitutions of these common peaks were analyzed using the UPLC-Q-TOF-MS system and nine of them were identified. In addition, LPS-stimulated RAW264.7 murine macrophages and DPPH assay were used to study the anti-inflammatory and anti-oxidation effects of D. genkwa. Then the fingerprint-efficacy relationships between UPLC fingerprints and pharmacodynamic data were studied with canonical correlation analysis. Analysis results indicated that the anti-inflammatory and anti-oxidation effects differed among the 10 D. genkwa samples owing to their inherent differences of chemical compositions. Taken together, this research established a fingerprint-efficacy relationship model of D. genkwa plant by combining the UPLC analytic technique and pharmacological research, which provided references for the detection of the principal components of traditional Chinese medicine on bioactivity.


Daphne genkwa; UPLC fingerprints; anti-inflammatory; anti-oxidant; canonical correlation analysis; fingerprint-efficacy relationship

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