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Br Poult Sci. 2017 Oct;58(5):490-497. doi: 10.1080/00071668.2017.1332403. Epub 2017 Jun 13.

Genetic diversity of mtDNA D-loop sequences in four native Chinese chicken breeds.

Author information

1
a Animal Genetics and Breeding, College of Animal Science and Veterinary Medicine , Henan Agricultural University, Henan Innovative Engineering Research Center of Poultry Germplasm Resource , Zhengzhou , PR China.

Abstract

1. To explore the genetic diversity of Chinese indigenous chicken breeds, a 585 bp fragment of the mitochondrial DNA (mtDNA) region was sequenced in 102 birds from the Xichuan black-bone chicken, Yunyang black-bone chicken and Lushi chicken. In addition, 30 mtDNA D-loop sequences of Silkie fowls were downloaded from NCBI. The mtDNA D-loop sequence polymorphism and maternal origin of 4 chicken breeds were analysed in this study. 2. The results showed that a total of 33 mutation sites and 28 haplotypes were detected in the 4 chicken breeds. The haplotype diversity and nucleotide diversity of these 4 native breeds were 0.916 ± 0.014 and 0.012 ± 0.002, respectively. Three clusters were formed in 4 Chinese native chickens and 12 reference breeds. Both the Xichuan black-bone chicken and Yunyang black-bone chicken were grouped into one cluster. Four haplogroups (A, B, C and E) emerged in the median-joining network in these breeds. 3. It was concluded that these 4 Chinese chicken breeds had high genetic diversity. The phylogenetic tree and median network profiles showed that Chinese native chickens and its neighbouring countries had at least two maternal origins, one from Yunnan, China and another from Southeast Asia or its surrounding area.

KEYWORDS:

Genetic diversity; indigenous chicken breeds; maternal origin; mtDNA D-loop region

PMID:
28541756
DOI:
10.1080/00071668.2017.1332403
[Indexed for MEDLINE]

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