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Lab Chip. 2017 Jun 13;17(12):2032-2045. doi: 10.1039/c7lc00241f.

Finding a helix in a haystack: nucleic acid cytometry with droplet microfluidics.

Author information

1
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA, USA. adam.abate@ucsf.edu.

Abstract

Nucleic acids encode the information of life, programming cellular functions and dictating many biological outcomes. Differentiating between cells based on their nucleic acid programs is, thus, a powerful way to unravel the genetic bases of many phenotypes. This is especially important considering that most cells exist in heterogeneous populations, requiring them to be isolated before they can be studied. Existing flow cytometry techniques, however, are unable to reliably recover specific cells based on nucleic acid content. Nucleic acid cytometry is a new field built on droplet microfluidics that allows robust identification, sorting, and sequencing of cells based on specific nucleic acid biomarkers. This review highlights applications that immediately benefit from the approach, biological questions that can be addressed for the first time with it, and considerations for building successful workflows.

PMID:
28540956
PMCID:
PMC6005652
DOI:
10.1039/c7lc00241f
[Indexed for MEDLINE]
Free PMC Article

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