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Methods Mol Biol. 2018;1697:153-171. doi: 10.1007/7651_2017_43.

Ceramide and S1P Signaling in Embryonic Stem Cell Differentiation.

Author information

1
Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
2
Department of Molecular and Cellular Medicine, Texas A&M Medical Health Sciences Center, Bryan, TX, USA.
3
Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA. ebieberich@augusta.edu.
4
Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, 1120 15th Street Room CA4012, Augusta, GA, 30912, USA. ebieberich@augusta.edu.

Abstract

Bioactive sphingolipids are important regulators for stem cell survival and differentiation. Most recently, we have coined the term "morphogenetic lipids" for sphingolipids that regulate stem cells during embryonic and postnatal development. The sphingolipid ceramide and its derivative, sphingosine-1-phosphate (S1P), can act synergistically as well as antagonistically on embryonic stem (ES) cell differentiation. We show here simple as well as state-of-the-art methods to analyze sphingolipids in differentiating ES cells and discuss new protocols to use ceramide and S1P analogs for the guided differentiation of mouse ES cells toward neuronal and glial lineage.

KEYWORDS:

Apoptosis; Ceramide; Neuroprogenitor; Oligodendrocyte precursor; Sphingolipid; Sphingosine-1-phosphate; Teratoma

PMID:
28540559
PMCID:
PMC5815858
DOI:
10.1007/7651_2017_43
[Indexed for MEDLINE]
Free PMC Article

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