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EMBO J. 1988 Dec 20;7(13):4045-53.

TnpA product encoded by the transposable element En-1 of Zea mays is a DNA binding protein.

Author information

1
Abteilung Molekulare Pflanzengenetik, Max-Planck-Institut für Züchtungsforschung, Köln, FRG.

Abstract

TnpA protein, the function encoded by the most abundant transcript of En-1 was expressed in Escherichia coli. DNA binding experiments with partially purified tnpA protein revealed that it binds to the subterminal repetitive region of En-1. TnpA protein recognizes a 12-bp-long sequence motif which is reiterated several times at the termini of En-1. Binding is reduced if the cytosine residues of CG dinucleotides and CNG trinucleotides within the motif are methylated. These data suggest a model in which the product of tnpA serves as a regulator of element activity.

PMID:
2854053
PMCID:
PMC455112
[Indexed for MEDLINE]
Free PMC Article

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