Send to

Choose Destination
EMBO J. 1988 Dec 20;7(13):4045-53.

TnpA product encoded by the transposable element En-1 of Zea mays is a DNA binding protein.

Author information

Abteilung Molekulare Pflanzengenetik, Max-Planck-Institut für Züchtungsforschung, Köln, FRG.


TnpA protein, the function encoded by the most abundant transcript of En-1 was expressed in Escherichia coli. DNA binding experiments with partially purified tnpA protein revealed that it binds to the subterminal repetitive region of En-1. TnpA protein recognizes a 12-bp-long sequence motif which is reiterated several times at the termini of En-1. Binding is reduced if the cytosine residues of CG dinucleotides and CNG trinucleotides within the motif are methylated. These data suggest a model in which the product of tnpA serves as a regulator of element activity.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center