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J Clin Microbiol. 2017 Aug;55(8):2445-2452. doi: 10.1128/JCM.00630-17. Epub 2017 May 24.

Rapid and Accurate Molecular Identification of the Emerging Multidrug-Resistant Pathogen Candida auris.

Author information

1
Public Health Research Institute, Rutgers Biomedical and Health Sciences, Newark, New Jersey, USA milena.kordalewska@rutgers.edu perlinds@njms.rutgers.edu.
2
Public Health Research Institute, Rutgers Biomedical and Health Sciences, Newark, New Jersey, USA.
3
Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
4
Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India.
5
Clínica El Rosario, Medellín, Colombia.
6
Medical and Experimental Mycology Group, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia.
7
Hospital General de Medellin Luz Castro de Gutiérrez ESE, Medellín, Colombia.

Abstract

Candida auris is an emerging multidrug-resistant fungal pathogen causing nosocomial and invasive infections associated with high mortality. C. auris is commonly misidentified as several different yeast species by commercially available phenotypic identification platforms. Thus, there is an urgent need for a reliable diagnostic method. In this paper, we present fast, robust, easy-to-perform and interpret PCR and real-time PCR assays to identify C. auris and related species: Candida duobushaemulonii, Candida haemulonii, and Candida lusitaniae Targeting rDNA region nucleotide sequences, primers specific for C. auris only or C. auris and related species were designed. A panel of 140 clinical fungal isolates was used in both PCR and real-time PCR assays followed by electrophoresis or melting temperature analysis, respectively. The identification results from the assays were 100% concordant with DNA sequencing results. These molecular assays overcome the deficiencies of existing phenotypic tests to identify C. auris and related species.

KEYWORDS:

Candida auris; Candida duobushaemulonii; Candida haemulonii; Candida lusitaniae; PCR; diagnostics; identification; real-time PCR

PMID:
28539346
PMCID:
PMC5527423
DOI:
10.1128/JCM.00630-17
[Indexed for MEDLINE]
Free PMC Article

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