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Bioresour Technol. 2017 Sep;239:454-463. doi: 10.1016/j.biortech.2017.04.071. Epub 2017 Apr 21.

Effects of adding trace elements on rice straw anaerobic mono-digestion: Focus on changes in microbial communities using high-throughput sequencing.

Author information

1
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
2
Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China.
3
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China. Electronic address: wxiaofen@cau.edu.cn.

Abstract

Although trace elements are known to aid anaerobic digestion, their mechanism of action is still unclear. High-throughput sequencing was used to reveal the mechanism by which adding trace elements affects microbial communities and their action. The results showed that the highest methane yields, with addition of Fe, Mo, Se and Mn were 289.2, 289.6, 285.3, 293.0mL/g volatile solids (VS), respectively. The addition of Fe, Mo, Se and Mn significantly (P<0.05) reduced the level of volatile fatty acids (VFAs). The dominant bacteria and archaea were Bacteroidetes and Methanosaeta, respectively. Compared with the proportion of Methanosaeta in the control group, treatment with added trace elements increased Methanosaeta by as much as 12.4%. Microbial community analysis indicated that adding trace elements changed the composition and diversity of archaea and bacteria. Methane yield was positively correlated with bacterial diversity and negatively correlated with archaeal diversity for most treatments.

KEYWORDS:

Anaerobic digestion; High-throughput sequencing; Microbial community; Trace element

PMID:
28538201
DOI:
10.1016/j.biortech.2017.04.071
[Indexed for MEDLINE]

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