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Eur Cell Mater. 2017 May 24;33:294-307. doi: 10.22203/eCM.v033a22.

Tendon healing induced by chemically modified mRNAs.

Author information

1
Ethris GmbH, Semmelweisstr. 3, 82152 Planegg, Germany.rudolph@ethris.com.

Abstract

Tendon disorders are frequent both in human and veterinary medicine with high re-injury rates and unsatisfactory therapeutic treatments. Application of naked, chemically-modified mRNA (cmRNA), encoding for therapeutic proteins, is an innovative approach to address tendon healing. In the current study, we demonstrated that injection of naked cmRNA, diluted in a glucose-containing solution, into tendons resulted in high protein expression in healthy and experimentally-injured tendons. Injection of bone morphogenetic protein 7 (BMP-7)-encoding cmRNA resulted in a significantly higher expression of BMP-7 protein and reduced formation of collagen type III, compared to vehicle control. Moreover, in a large animal model, reporter protein expression was detectable not only in healthy, but also in experimentally-injured, severely inflamed tendons. Summarising, these results demonstrated the potential of cmRNAs encoding for therapeutic proteins as a new class of drugs for the treatment of tendon disorders.

PMID:
28537650
DOI:
10.22203/eCM.v033a22
[Indexed for MEDLINE]
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