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Elife. 2017 May 24;6. pii: e23623. doi: 10.7554/eLife.23623.

The dynamic three-dimensional organization of the diploid yeast genome.

Author information

1
Department of Genome Sciences, University of Washington, Seattle, United States.
2
Department of Molecular Biosciences, Northwestern University, Evanston, United States.
3
Howard Hughes Medical Institute, University of Washington, Seattle, United States.

Abstract

The budding yeast Saccharomyces cerevisiae is a long-standing model for the three-dimensional organization of eukaryotic genomes. However, even in this well-studied model, it is unclear how homolog pairing in diploids or environmental conditions influence overall genome organization. Here, we performed high-throughput chromosome conformation capture on diverged Saccharomyces hybrid diploids to obtain the first global view of chromosome conformation in diploid yeasts. After controlling for the Rabl-like orientation using a polymer model, we observe significant homolog proximity that increases in saturated culture conditions. Surprisingly, we observe a localized increase in homologous interactions between the HAS1-TDA1 alleles specifically under galactose induction and saturated growth. This pairing is accompanied by relocalization to the nuclear periphery and requires Nup2, suggesting a role for nuclear pore complexes. Together, these results reveal that the diploid yeast genome has a dynamic and complex 3D organization.

KEYWORDS:

Hi-C; S. cerevisiae; S. paradoxus; S. uvarum; chromosome organization; chromosomes; evolutionary biology; genes; genomics; homolog pairing; nuclear organization

PMID:
28537556
PMCID:
PMC5476426
DOI:
10.7554/eLife.23623
[Indexed for MEDLINE]
Free PMC Article

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