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Elife. 2017 May 24;6. pii: e23623. doi: 10.7554/eLife.23623.

The dynamic three-dimensional organization of the diploid yeast genome.

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Department of Genome Sciences, University of Washington, Seattle, United States.
Department of Molecular Biosciences, Northwestern University, Evanston, United States.
Howard Hughes Medical Institute, University of Washington, Seattle, United States.


The budding yeast Saccharomyces cerevisiae is a long-standing model for the three-dimensional organization of eukaryotic genomes. However, even in this well-studied model, it is unclear how homolog pairing in diploids or environmental conditions influence overall genome organization. Here, we performed high-throughput chromosome conformation capture on diverged Saccharomyces hybrid diploids to obtain the first global view of chromosome conformation in diploid yeasts. After controlling for the Rabl-like orientation using a polymer model, we observe significant homolog proximity that increases in saturated culture conditions. Surprisingly, we observe a localized increase in homologous interactions between the HAS1-TDA1 alleles specifically under galactose induction and saturated growth. This pairing is accompanied by relocalization to the nuclear periphery and requires Nup2, suggesting a role for nuclear pore complexes. Together, these results reveal that the diploid yeast genome has a dynamic and complex 3D organization.


Hi-C; S. cerevisiae; S. paradoxus; S. uvarum; chromosome organization; chromosomes; evolutionary biology; genes; genomics; homolog pairing; nuclear organization

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