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Elife. 2017 May 31;6. pii: e26652. doi: 10.7554/eLife.26652.

Longitudinal imaging of Caenorhabditis elegans in a microfabricated device reveals variation in behavioral decline during aging.

Author information

1
Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, United States.
2
Department of Neurology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.
3
Department of Neuroscience, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States.

Abstract

The roundworm C. elegans is a mainstay of aging research due to its short lifespan and easily manipulable genetics. Current, widely used methods for long-term measurement of C. elegans are limited by low throughput and the difficulty of performing longitudinal monitoring of aging phenotypes. Here we describe the WorMotel, a microfabricated device for long-term cultivation and automated longitudinal imaging of large numbers of C. elegans confined to individual wells. Using the WorMotel, we find that short-lived and long-lived strains exhibit patterns of behavioral decline that do not temporally scale between individuals or populations, but rather resemble the shortest and longest lived individuals in a wild type population. We also find that behavioral trajectories of worms subject to oxidative stress resemble trajectories observed during aging. Our method is a powerful and scalable tool for analysis of C. elegans behavior and aging.

KEYWORDS:

C. elegans; WorMotel; aging; behavior; chromosomes; genes; healthspan; lifespan; neuroscience; stress resistance

PMID:
28537553
PMCID:
PMC5484621
DOI:
10.7554/eLife.26652
[Indexed for MEDLINE]
Free PMC Article

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