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Bioresour Technol. 2017 Sep;239:394-401. doi: 10.1016/j.biortech.2017.04.070. Epub 2017 Apr 21.

Efficient bioconversion of high-content volatile fatty acids into microbial lipids by Cryptococcus curvatus ATCC 20509.

Author information

1
College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, The Collaborative Innovation Center For Regional Environmental Quality, Tongji University, Shanghai 200092, China.
2
College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse, Ministry of Education Key Laboratory of Yangtze River Water Environment, The Collaborative Innovation Center For Regional Environmental Quality, Tongji University, Shanghai 200092, China. Electronic address: liujia@tongji.edu.cn.

Abstract

The lower utilization ratios of high-content VFAs resulted in less lipid yield by the oleaginous yeast Cryptococcus curvatus ATCC 20509. In this work, increasing the inoculation concentration to OD600=5.1 improved the acetic acid utilization ratio (99.8%) and lipids production (4.63g/L) in mediums with 30g/L of acetic acid. For the higher acetic acid concentration (40g/L), increasing the nitrogen to carbon ratio (0.033) and raising the initial pH (pH=8) was superior over improvement in the inoculate, with the lipid production increased from 1.08g/L to 6.49g/L. Subsequently, mixed VFAs at concentrations of 30g/L and 40g/L were used as the carbon source to simulate waste-derived VFAs. High lipid production (4.82 and 7.45g/L, respectively) was correspondingly achieved with similar high lipid yield (0.187g/g). This study provides an effective strategy to enhance the bioconversion of high-content VFAs into microbial lipids.

KEYWORDS:

C. curvatus ATCC 20509; Initial pH; Inoculation concentration; Microbial lipids; VFAs

PMID:
28535491
DOI:
10.1016/j.biortech.2017.04.070
[Indexed for MEDLINE]

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