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Environ Sci Technol. 2017 Jun 20;51(12):6745-6755. doi: 10.1021/acs.est.7b00856. Epub 2017 Jun 8.

Ecophysiological Examination of the Lake Erie Microcystis Bloom in 2014: Linkages between Biology and the Water Supply Shutdown of Toledo, OH.

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Department of Biology, James Madison University , Harrisonburg, Virginia 22807, United States.
NOAA-GLERL, 4840 South State Rd., Ann Arbor, Michigan 48108, United States.
Department of Biological Sciences, Bowling Green State University , Bowling Green, Ohio 43403, United States.
Department of Microbiology, University of Tennessee , Knoxville, Tennessee 37996, United States.
Department of Chemistry, State University of New York, Environmental Science and Forestry , Syracuse, New York 13210, United States.
Cooperative Institute for Limnology and Ecosystems Research, University of Michigan , Ann Arbor, Michigan 48108, United States.
Department of Earth and Environmental Sciences, University of Michigan , Ann Arbor, Michigan 48109, United States.
Genomic Service Laboratory, Hudson Alpha Institute for Biotechnology , Huntsville, Alabama 35806, United States.
NOAA National Ocean Service, National Centers for Coastal Ocean Sciences, Silver Spring, Maryland 20910, United States.
Department of Life Sciences, Texas A&M Corpus Christi , Corpus Christi, Texas 78412, United States.


Annual cyanobacterial blooms dominated by Microcystis have occurred in western Lake Erie (U.S./Canada) during summer months since 1995. The production of toxins by bloom-forming cyanobacteria can lead to drinking water crises, such as the one experienced by the city of Toledo in August of 2014, when the city was rendered without drinking water for >2 days. It is important to understand the conditions and environmental cues that were driving this specific bloom to provide a scientific framework for management of future bloom events. To this end, samples were collected and metatranscriptomes generated coincident with the collection of environmental metrics for eight sites located in the western basin of Lake Erie, including a station proximal to the water intake for the city of Toledo. These data were used to generate a basin-wide ecophysiological fingerprint of Lake Erie Microcystis populations in August 2014 for comparison to previous bloom communities. Our observations and analyses indicate that, at the time of sample collection, Microcystis populations were under dual nitrogen (N) and phosphorus (P) stress, as genes involved in scavenging of these nutrients were being actively transcribed. Targeted analysis of urea transport and hydrolysis suggests a potentially important role for exogenous urea as a nitrogen source during the 2014 event. Finally, simulation data suggest a wind event caused microcystin-rich water from Maumee Bay to be transported east along the southern shoreline past the Toledo water intake. Coupled with a significant cyanophage infection, these results reveal that a combination of biological and environmental factors led to the disruption of the Toledo water supply. This scenario was not atypical of reoccurring Lake Erie blooms and thus may reoccur in the future.

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