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J Infect Dis. 2017 Jul 1;216(1):117-124. doi: 10.1093/infdis/jix254.

Live Attenuated Pertussis Vaccine BPZE1 Protects Baboons Against Bordetella pertussis Disease and Infection.

Locht C1,2,3,4, Papin JF5, Lecher S1,2,3,4, Debrie AS1,2,3,4, Thalen M6, Solovay K6, Rubin K6, Mielcarek N1,2,3,4.

Author information

1
Institut Pasteur de Lille, Center for Infection and Immunity of Lille.
2
University of Lille.
3
Centre National de la Recherche Scientifique, UMR 8204.
4
Institut National de la Santé et de la Recherche Médicale (INSERM), U1019, Lille, France.
5
University of Oklahoma Health Sciences Center, Oklahoma Baboon Research Resource.
6
ILiAD Biotechnologies, New York.

Abstract

Evidence suggests that the resurgence of pertussis in many industrialized countries may result from the failure of current vaccines to prevent nasopharyngeal colonization by Bordetella pertussis, the principal causative agent of whooping cough. Here, we used a baboon model to test the protective potential of the novel, live attenuated pertussis vaccine candidate BPZE1. A single intranasal/intratracheal inoculation of juvenile baboons with BPZE1 resulted in transient nasopharyngeal colonization and induction of immunoglobulin G and immunoglobulin A to all antigens tested, while causing no adverse symptoms or leukocytosis. When BPZE1-vaccinated baboons were challenged with a high dose of a highly virulent B. pertussis isolate, they were fully protected against disease, whereas naive baboons developed illness (with 1 death) and leukocytosis. Total postchallenge nasopharyngeal virulent bacterial burden of vaccinated animals was substantially reduced (0.002%) compared to naive controls, providing promising evidence in nonhuman primates that BPZE1 protects against both pertussis disease and B. pertussis infection.

KEYWORDS:

baboon model; live attenuated vaccine; pertussis

PMID:
28535276
PMCID:
PMC5853371
DOI:
10.1093/infdis/jix254
[Indexed for MEDLINE]
Free PMC Article

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