Format

Send to

Choose Destination
Sci Rep. 2017 May 22;7(1):2221. doi: 10.1038/s41598-017-01459-z.

Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA.

Author information

1
Department of NMR & MRI Facility, All India Institute of Medical Sciences, New Delhi, 110029, India.
2
Department of Biophysics, All India Institute of Medical Sciences, New Delhi, 110029, India.
3
Department of NMR & MRI Facility, All India Institute of Medical Sciences, New Delhi, 110029, India. umasharma69@gmail.com.
4
Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029, India.
5
Department of Surgical Disciplines, All India Institute of Medical Sciences, New Delhi, 110029, India.
6
Department of NMR & MRI Facility, All India Institute of Medical Sciences, New Delhi, 110029, India. jagan1954@hotmail.com.

Abstract

Total choline (tCho) was documented as a biomarker for breast cancer diagnosis by in vivo MRS. To understand the molecular mechanisms behind elevated tCho in breast cancer, an association of tCho with β-catenin and cyclin D1 was evaluated. Hundred fractions from 20 malignant, 10 benign and 20 non-involved breast tissues were isolated. Cytosolic and nuclear expressions of β-catenin and cyclin D1 were estimated using ELISA. Higher tCho was seen in malignant compared to benign tissues. Malignant tissues showed higher cytosolic and nuclear β-catenin expressions than benign and non-involved tissues. Within malignant tissues, β-catenin and cyclin D1 expressions were higher in the nucleus than cytosol. Cyclin D1 expression was higher in the cytosolic fractions of benign and non-involved than malignant tissues. Furthermore, in malignant tissues, tCho showed a positive correlation with the cytosolic and nuclear expression of β-catenin and cyclin D1 and also a correlation between nuclear expressions of both these proteins was seen. Higher cytosolic β-catenin expression was seen in progesterone receptor negative than positive patients. Results provide an evidence of correlation between non-invasive biomarker, tCho and the Wnt/β-catenin pathway. The findings explain the molecular mechanism of tCho elevation which may facilitate exploration of additional therapeutic targets for breast cancer.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center