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Elife. 2017 May 22;6. pii: e25364. doi: 10.7554/eLife.25364.

A genetic basis for molecular asymmetry at vertebrate electrical synapses.

Author information

1
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, United States.
2
Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, United States.
3
Department of Ophthalmology and Visual Science, McGovern Medical School, University of Texas Health Sciences Center at Houston, Houston, United States.

Abstract

Neural network function is based upon the patterns and types of connections made between neurons. Neuronal synapses are adhesions specialized for communication and they come in two types, chemical and electrical. Communication at chemical synapses occurs via neurotransmitter release whereas electrical synapses utilize gap junctions for direct ionic and metabolic coupling. Electrical synapses are often viewed as symmetrical structures, with the same components making both sides of the gap junction. By contrast, we show that a broad set of electrical synapses in zebrafish, Danio rerio, require two gap-junction-forming Connexins for formation and function. We find that one Connexin functions presynaptically while the other functions postsynaptically in forming the channels. We also show that these synapses are required for the speed and coordination of escape responses. Our data identify a genetic basis for molecular asymmetry at vertebrate electrical synapses and show they are required for appropriate behavioral performance.

KEYWORDS:

behavior; connexins; electrical synapse; neural circuit wiring; neuroscience; synaptogenesis; zebrafish

PMID:
28530549
PMCID:
PMC5462537
DOI:
10.7554/eLife.25364
[Indexed for MEDLINE]
Free PMC Article

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