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Nat Commun. 2017 May 22;8:15362. doi: 10.1038/ncomms15362.

Thermogenetic neurostimulation with single-cell resolution.

Author information

1
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
2
Pirogov Russian National Research Medical University, Moscow 117997, Russia.
3
Physics Department, International Laser Center, M.V. Lomonosov Moscow State University, Moscow 119992, Russia.
4
Department of Physics and Astronomy, Texas A&M University, College Station, Texas 77843, USA.
5
Russian Quantum Center, ul. Novaya 100, Skolkovo, Moscow Region 143025, Russia.
6
Kazan Quantum Center, A.N. Tupolev Kazan National Research Technical University, 420126 Kazan, Russia.
7
Kurchatov Institute National Research Center, Moscow 123182, Russia.
8
Institute of Higher Nervous Activity and Neurophysiology, Moscow 117485, Russia.
9
Institute for Cardiovascular Physiology, Georg August University Göttingen, D-37075 Göttingen, Germany.

Abstract

Thermogenetics is a promising innovative neurostimulation technique, which enables robust activation of neurons using thermosensitive transient receptor potential (TRP) cation channels. Broader application of this approach in neuroscience is, however, hindered by a limited variety of suitable ion channels, and by low spatial and temporal resolution of neuronal activation when TRP channels are activated by ambient temperature variations or chemical agonists. Here, we demonstrate rapid, robust and reproducible repeated activation of snake TRPA1 channels heterologously expressed in non-neuronal cells, mouse neurons and zebrafish neurons in vivo by infrared (IR) laser radiation. A fibre-optic probe that integrates a nitrogen-vacancy (NV) diamond quantum sensor with optical and microwave waveguide delivery enables thermometry with single-cell resolution, allowing neurons to be activated by exceptionally mild heating, thus preventing the damaging effects of excessive heat. The neuronal responses to the activation by IR laser radiation are fully characterized using Ca2+ imaging and electrophysiology, providing, for the first time, a complete framework for a thermogenetic manipulation of individual neurons using IR light.

PMID:
28530239
PMCID:
PMC5493594
DOI:
10.1038/ncomms15362
[Indexed for MEDLINE]
Free PMC Article

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