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Fitoterapia. 2017 Jul;120:17-24. doi: 10.1016/j.fitote.2017.05.004. Epub 2017 May 18.

Anti-inflammatory activities of compounds from twigs of Morus alba.

Author information

1
College of Pharmacy, Catholic University of Daegu, Gyeongbuk 38430, Republic of Korea.
2
College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 41566, Republic of Korea. Electronic address: jkim6923@knu.ac.kr.
3
Korean Medicine of College, Daegu Haany University, Daegu 42158, Republic of Korea.
4
Department of Food and Life Science, Pukyong National University, Busan 48513, Republic of Korea.
5
Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, Gangwon-Do 24341, Republic of Korea.
6
College of Pharmacy, Catholic University of Daegu, Gyeongbuk 38430, Republic of Korea. Electronic address: bsmin@cu.ac.kr.

Abstract

Five new compounds, 10-oxomornigrol F (1), (7″R)-(-)-6-(7″-hydroxy-3″,8″-dimethyl-2″,8″-octadien-1″-yl)apigenin (2), ramumorin A (3), ramumorin B (4), and (4S,7S,8R)-trihydroxyoctadeca-5Z-enoic acid (5), together with 31 known compounds (6-36), were isolated from the twigs of Morus alba (Moraceae). The chemical structures of these compounds were established using spectroscopic analyses, 1D and 2D NMR, high-resolution electrospray ionization mass spectrometry (HRESIMS), and Mosher's methods. The anti-inflammatory activities of the compounds were evaluated by investigating their ability to inhibit lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW 264.7 cells. Compounds 1, 2, 13, 17, 19, 25-28, and 32 showed inhibitory effects with IC50 values ranging from 2.2 to 5.3μg/mL. Compounds 1, 2, 17, 25, and 32 reduced LPS-induced inducible nitric oxide synthase (iNOS) expression in a concentration-dependent manner. In addition, pretreating the cells with compound 1, 17, and 32 significantly suppressed LPS-induced expression of cyclooxygenase-2 (COX-2) protein.

KEYWORDS:

Anti-inflammatory activity; Flavonoid; Moraceae; Morus alba; NO inhibition

PMID:
28529178
DOI:
10.1016/j.fitote.2017.05.004
[Indexed for MEDLINE]

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