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Am J Hum Genet. 2017 Jun 1;100(6):954-959. doi: 10.1016/j.ajhg.2017.04.013. Epub 2017 May 18.

Pleiotropic Effects of Trait-Associated Genetic Variation on DNA Methylation: Utility for Refining GWAS Loci.

Author information

1
University of Exeter Medical School, Exeter EX2 5DW, UK.
2
MRC Centre for Neuropsychiatric Genetics and Genomics, Cardiff University School of Medicine, Cardiff CF24 4HQ, UK.
3
University of Exeter Medical School, Exeter EX2 5DW, UK. Electronic address: j.mill@exeter.ac.uk.

Abstract

Most genetic variants identified in genome-wide association studies (GWASs) of complex traits are thought to act by affecting gene regulation rather than directly altering the protein product. As a consequence, the actual genes involved in disease are not necessarily the most proximal to the associated variants. By integrating data from GWAS analyses with those from genetic studies of regulatory variation, it is possible to identify variants pleiotropically associated with both a complex trait and measures of gene regulation. In this study, we used summary-data-based Mendelian randomization (SMR), a method developed to identify variants pleiotropically associated with both complex traits and gene expression, to identify variants associated with complex traits and DNA methylation. We used large DNA methylation quantitative trait locus (mQTL) datasets generated from two different tissues (blood and fetal brain) to prioritize genes for >40 complex traits with robust GWAS data and found considerable overlap with the results of SMR analyses performed with expression QTL (eQTL) data. We identified multiple examples of variable DNA methylation associated with GWAS variants for a range of complex traits, demonstrating the utility of this approach for refining genetic association signals.

KEYWORDS:

DNA methylation; GWAS; blood; brain; complex trait; disease; epigenetics; genetics; genome-wide association study; pleiotropy

PMID:
28528868
PMCID:
PMC5473725
[Available on 2017-12-01]
DOI:
10.1016/j.ajhg.2017.04.013
[Indexed for MEDLINE]
Free PMC Article

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