Format

Send to

Choose Destination
Colloids Surf B Biointerfaces. 2017 Aug 1;156:104-113. doi: 10.1016/j.colsurfb.2017.05.016. Epub 2017 May 9.

Incorporation of silver nanoparticles into magnetron-sputtered calcium phosphate layers on titanium as an antibacterial coating.

Author information

1
Centre of Technology, Department of Experimental Physics, Lenin Avenue 43, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.
2
Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), Universitaetsstr, 5-7, University of Duisburg-Essen, 45117 Essen, Germany.
3
Material Properties Measurements Centre, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia.
4
Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), Universitaetsstr, 5-7, University of Duisburg-Essen, 45117 Essen, Germany. Electronic address: matthias.epple@uni-due.de.
5
Centre of Technology, Department of Experimental Physics, Lenin Avenue 43, National Research Tomsk Polytechnic University, 634050 Tomsk, Russia. Electronic address: rsurmenev@mail.ru.

Abstract

A three-layer system of nanocrystalline hydroxyapatite (first layer; 1000nm thick), silver nanoparticles (second layer; 1.5μg Ag cm-2) and calcium phosphate (third layer, either 150 or 1000nm thick) on titanium was prepared by a combination of electrophoretic deposition of silver nanoparticles and the deposition of calcium phosphate by radio frequency magnetron sputtering. Scanning electron microscopy showed that the silver nanoparticles were evenly distributed over the surface. The adhesion of multilayered coating on the substrate was evaluated using the scratch test method. The resistance to cracking and delamination indicated that the multilayered coating has good resistance to contact damage. The release of silver ions from the hydroxyapatite/silver nanoparticle/calcium phosphate system into the phosphate-buffered saline (PBS) solution was measured by atomic absorption spectroscopy (AAS). Approximately one-third of the incorporated silver was released after 3days immersion into PBS, indicating a total release time of the order of weeks. There were no signs of cracks on the surface of the coating after immersion after various periods, indicating the excellent mechanical stability of the multilayered coating in the physiological environment. An antimicrobial effect against Escherichia coli was found for a 150nm thick outer layer of the calcium phosphate using a semi-quantitative turbidity test.

KEYWORDS:

Antibacterial coatings; Calcium phosphate; Coatings; Electrophoretic deposition; Radio frequency magnetron sputtering; Silver

PMID:
28527354
DOI:
10.1016/j.colsurfb.2017.05.016
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center