Format

Send to

Choose Destination
Curr Opin Chem Biol. 2017 Oct;40:24-30. doi: 10.1016/j.cbpa.2017.04.015. Epub 2017 May 17.

Harnessing plant metabolic diversity.

Author information

1
Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
2
Engineering Biology, The Earlham Institute, Norwich Research Park, Norwich NR4 7UZ, UK.
3
Department of Metabolic Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Electronic address: anne.osbourn@jic.ac.uk.

Abstract

Advances in DNA sequencing and synthesis technologies in the twenty-first century are now making it possible to build large-scale pipelines for engineering plant natural product pathways into heterologous production species using synthetic biology approaches. The ability to decode the chemical potential of plants by sequencing their transcriptomes and/or genomes and to then use this information as an instruction manual to make drugs and other high-value chemicals is opening up new routes to harness the vast chemical diversity of the Plant Kingdom. Here we describe recent progress in methods for pathway discovery, DNA synthesis and assembly, and expression of engineered pathways in heterologous hosts. We also highlight the importance of standardization and the challenges associated with dataset integration in the drive to build a systematic framework for effective harnessing of plant metabolic diversity.

PMID:
28527344
PMCID:
PMC5693780
DOI:
10.1016/j.cbpa.2017.04.015
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center