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Biotechnol Lett. 2017 Aug;39(8):1237-1244. doi: 10.1007/s10529-017-2357-7. Epub 2017 May 19.

A new maltose-inducible high-performance heterologous expression system in Bacillus subtilis.

Yue J1,2,3, Fu G2,3, Zhang D4,5, Wen J1.

Author information

1
Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.
2
Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.
3
Key Laboratories of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.
4
Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China. zhang_dw@tib.cas.cn.
5
Key Laboratories of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China. zhang_dw@tib.cas.cn.

Abstract

OBJECTIVES:

To improve heterologous proteins production, we constructed a maltose-inducible expression system in Bacillus subtilis.

RESULTS:

An expression system based on the promoter for maltose utilization constructed in B. subtilis. Successively, to improve the performance of the P malA -derived system, mutagenesis was employed by gradually shortening the length of P malA promoter and altering the spacing between the predicted MalR binding site and the -35 region. Furthermore, deletion of the maltose utilization genes (malL and yvdK) improved the P malA promoter activity. Finally, using this efficient maltose-inducible expression system, we enhanced the production of luciferase and D-aminoacylase, compared with the P hpaII system.

CONCLUSIONS:

A maltose-inducible expression system was constructed and evaluated. It could be used for high level expression of heterologous proteins production.

KEYWORDS:

Bacillus subtilis; D-Aminoacylase; Expression vector; Gene deletion; Luciferase; P malA promoter

PMID:
28527120
DOI:
10.1007/s10529-017-2357-7
[Indexed for MEDLINE]

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