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Chromosoma. 2017 Dec;126(6):681-695. doi: 10.1007/s00412-017-0631-z. Epub 2017 May 19.

The PRDM9 KRAB domain is required for meiosis and involved in protein interactions.

Author information

1
Institut de Génétique Humaine UMR9002 CNRS-Université de Montpellier, 141 rue de la cardonille, 34396, Montpellier cedex 05, France.
2
SEAT-TAAM CNRS Phenomin UPS44, 7 rue Guy Môquet, 94800, Villejuif, France.
3
Faculty of Medicine at the TU Dresden, Institute of Physiological Chemistry, Fetscherstraße 74, 01307, Dresden, Germany.
4
Institut de Génétique Humaine UMR9002 CNRS-Université de Montpellier, 141 rue de la cardonille, 34396, Montpellier cedex 05, France. bernard.de-massy@igh.cnrs.fr.

Abstract

PR domain-containing protein 9 (PRDM9) is a major regulator of the localization of meiotic recombination hotspots in the human and mouse genomes. This role involves its DNA-binding domain, which is composed of a tandem array of zinc fingers, and PRDM9-dependent trimethylation of histone H3 at lysine 4. PRDM9 is a member of the PRDM family of transcription regulators, but unlike other family members, it contains a Krüppel-associated box (KRAB)-related domain that is predicted to be a potential protein interaction domain. Here, we show that truncation of the KRAB domain of mouse PRDM9 leads to loss of PRDM9 function and altered meiotic prophase and gametogenesis. In addition, we identified proteins that interact with the KRAB domain of PRDM9 in yeast two-hybrid assay screens, particularly CXXC1, a member of the COMPASS complex. We also show that CXXC1 interacts with IHO1, an essential component of the meiotic double-strand break (DSB) machinery. As CXXC1 is orthologous to Saccharomyces cerevisiae Spp1 that links DSB sites to the DSB machinery on the chromosome axis, we propose that these molecular interactions involved in the regulation of meiotic DSB formation are conserved in mouse meiosis.

KEYWORDS:

CXXC1; IHO1; Meiosis; PRDM9 KRAB domain; Protein interactions; Recombination

PMID:
28527011
PMCID:
PMC5688218
DOI:
10.1007/s00412-017-0631-z
[Indexed for MEDLINE]
Free PMC Article

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