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Antioxidants (Basel). 2017 May 18;6(2). pii: E35. doi: 10.3390/antiox6020035.

Green Synthesized Zinc Oxide (ZnO) Nanoparticles Induce Oxidative Stress and DNA Damage in Lathyrus sativus L. Root Bioassay System.

Author information

1
Molecular Biology and Genomics Laboratory, Department of Botany, Berhampur University, Berhampur 760007, Odisha, India. kamalpanda51@gmail.com.
2
Molecular Biology and Genomics Laboratory, Department of Botany, Berhampur University, Berhampur 760007, Odisha, India. dambarugolari@gmail.com.
3
Molecular Biology and Genomics Laboratory, Department of Botany, Berhampur University, Berhampur 760007, Odisha, India. venu86job@gmail.com.
4
Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India. achary.mohan1@gmail.com.
5
Material Chemistry Laboratory, Department of Chemistry, Berhampur University, Berhampur 760007, Odisha, India. g.phaomei@gmail.com.
6
Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine, Davis Heart and Lung Research Institute, Ohio State University College of Medicine, Columbus, OH 43210, USA. narasimham.parinandi@osumc.edu.
7
Condensed Matter Physics Division, Indira Gandhi Centre for Atomic Research, Kalpakum, Tamil Nadu 603102, India. sahuhk@gmail.com.
8
Molecular Biology and Genomics Laboratory, Department of Botany, Berhampur University, Berhampur 760007, Odisha, India. panda.brahma@gmail.com.

Abstract

Zinc oxide nanoparticles (ZnONP-GS) were synthesised from the precursor zinc acetate (Zn(CH₃COO)₂) through the green route using the milky latex from milk weed (Calotropis gigantea L. R. Br) by alkaline precipitation. Formation of the ZnONP-GS was monitored by UV-visible spectroscopy followed by characterization and confirmation by energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and X-ray diffraction (XRD). Both the ZnONP-GS and the commercially available ZnONP-S (Sigma-Aldrich) and cationic Zn2+ from Zn(CH₃COO)₂ were tested in a dose range of 0-100 mg·L-1 for their potency (i) to induce oxidative stress as measured by the generation reactive oxygen species (ROS: O₂•-, H₂O₂ and OH), cell death, and lipid peroxidation; (ii) to modulate the activities of antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD), guaiacol peroxidase (GPX), and ascorbate peroxidase (APX); and (iii) to cause DNA damage as determined by Comet assay in Lathyrus sativus L. root bioassay system. Antioxidants such as Tiron and dimethylthiourea significantly attenuated the ZnONP-induced oxidative and DNA damage, suggesting the involvement of ROS therein. Our study demonstrated that both ZnONP-GS and ZnONP-S induced oxidative stress and DNA damage to a similar extent but were significantly less potent than Zn2+ alone.

KEYWORDS:

Calotropis gigantea; Lathyrus sativus root; comet assay; green synthesis; oxidative stress; plant bioassay; reactive oxygen species; zinc oxide nanoparticles

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