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ACS Appl Mater Interfaces. 2017 Jun 7;9(22):18423-18428. doi: 10.1021/acsami.7b03905. Epub 2017 May 30.

Peptide-Functionalized Nanomaterials for the Efficient Isolation of HER2-Positive Circulating Tumor Cells.

Author information

1
Department of Chemistry, Renmin University of China , Beijing 100872, China.
2
CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology , Beijing 100190, China.
3
Department of Breast Oncology, Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute , 52 Fucheng Rd, Beijing 100142, China.
4
University of Chinese Academy of Sciences , Beijing 100049, China.

Abstract

The detection of circulating tumor cells (CTCs) with a specific antigen expression is necessary in therapeutic response monitoring and targeted therapy guidance. The existence of human epidermal growth factor receptor 2 (HER2) and the concentration of HER2-positive CTCs are strong indicators for patient diagnosis, prognosis, and therapeutic monitoring. Herein we report the direct isolation of HER2-positive CTCs by peptide-functionalized nanomaterials. We designed and screened out a peptide as an HER2 antibody alternative demonstrating high HER2 affinity and selectivity. This HER2 recognition peptide bound efficiently with HER2 at the ligand-binding domain. Efficient HER2-positive CTC capture and detection were demonstrated using magnetic nanoparticles functionalized with the HER2 recognition peptide.

KEYWORDS:

HER2; cancer metastasis; circulating tumor cells; nanomaterials; peptide

PMID:
28523908
DOI:
10.1021/acsami.7b03905
[Indexed for MEDLINE]

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