Efficient production of 2,3-butanediol by recombinant Saccharomyces cerevisiae through modulation of gene expression by cocktail δ-integration

Ryosuke Yamada; Kazuki Wakita; Ryosuke Mitsui; Riru Nishikawa; Hiroyasu Ogino

doi:10.1016/j.biortech.2017.05.034

Efficient production of 2,3-butanediol by recombinant Saccharomyces cerevisiae through modulation of gene expression by cocktail δ-integration

Bioresour Technol. 2017 Dec;245(Pt B):1558-1566. doi: 10.1016/j.biortech.2017.05.034. Epub 2017 May 10.

Authors

Ryosuke Yamada¹, Kazuki Wakita², Ryosuke Mitsui³, Riru Nishikawa⁴, Hiroyasu Ogino⁵

Affiliations

¹ Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. Electronic address: yamada@chemeng.osakafu-u.ac.jp.
² Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. Electronic address: kwakita@chemeng.osakafu-u.ac.jp.
³ Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. Electronic address: rmitsui@chemeng.osakafu-u.ac.jp.
⁴ Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. Electronic address: rnishikawa@chemeng.osakafu-u.ac.jp.
⁵ Department of Chemical Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, Japan. Electronic address: ogino@chemeng.osakafu-u.ac.jp.

PMID: 28522198
DOI: 10.1016/j.biortech.2017.05.034

Abstract

In this study, the expression of 4 genes encoding α-acetolactate synthase, α-acetolactate decarboxylase, 2,3-butanediol dehydrogenase, and NADH oxidase was modulated using a previously developed cocktail δ-integration strategy. The resultant strain, YPH499/dPdAdG/BD6-10, was used in a fed-batch cultivation for the production of 2,3-butanediol. The concentration, production rate, and yield obtained were 80.0g/L, 4.00g/L/h, and 41.7%, respectively. The production rate and yield of the compound obtained are higher for this strain compared to reports published for Saccharomyces cerevisiae so far. The cocktail δ-integration strategy allows for modulation of multiple gene expression, without the exact knowledge of rate-limiting steps, and therefore, could be used as a promising strategy for the production of bio-based chemicals in recombinant S. cerevisiae.

Keywords: 2,3-Butanediol; Fed-batch; Fermentation; Metabolic engineering; Saccharomyces cerevisiae.

MeSH terms

Butylene Glycols*
Carboxy-Lyases
Fermentation
Metabolic Engineering*
Saccharomyces cerevisiae*

Substances

Butylene Glycols
2,3-butylene glycol
Carboxy-Lyases
acetolactate decarboxylase