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Gut Pathog. 2017 May 15;9:29. doi: 10.1186/s13099-017-0175-z. eCollection 2017.

Comparative genomic analysis reveals genetic features related to the virulence of Bacillus cereus FORC_013.

Koo HJ1, Ahn S2,3, Chung HY3,4, Kim S3,4, Kim K2, Ryu S3,4, Lee JH3,5, Choi SH3,4, Kim H1,2,3.

Author information

1
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
2
Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul, Republic of Korea.
3
Food-Borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul, Republic of Korea.
4
Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul, Republic of Korea.
5
Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin, Republic of Korea.

Abstract

BACKGROUND:

Bacillus cereus is well known as a gastrointestinal pathogen that causes food-borne illness. In the present study, we sequenced the complete genome of B. cereus FORC_013 isolated from fried eel in South Korea. To extend our understanding of the genomic characteristics of FORC_013, we conducted a comparative analysis with the published genomes of other B. cereus strains.

RESULTS:

We fully assembled the single circular chromosome (5,418,913 bp) and one plasmid (259,749 bp); 5511 open reading frames (ORFs) and 283 ORFs were predicted for the chromosome and plasmid, respectively. Moreover, we detected that the enterotoxin (NHE, HBL, CytK) induces food-borne illness with diarrheal symptom, and that the pleiotropic regulator, along with other virulence factors, plays a role in surviving and biofilm formation. Through comparative analysis using the complete genome sequence of B. cereus FORC_013, we identified both positively selected genes related to virulence regulation and 224 strain-specific genes of FORC_013.

CONCLUSIONS:

Through genome analysis of B. cereus FORC_013, we identified multiple virulence factors that may contribute to pathogenicity. These results will provide insight into further studies regarding B. cereus pathogenesis mechanism at the genomic level.

KEYWORDS:

Bacillus cereus; Comparative genomics; Positive selection; Unique genes; Virulence

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