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Gut Pathog. 2017 May 15;9:29. doi: 10.1186/s13099-017-0175-z. eCollection 2017.

Comparative genomic analysis reveals genetic features related to the virulence of Bacillus cereus FORC_013.

Koo HJ1, Ahn S2,3, Chung HY3,4, Kim S3,4, Kim K2, Ryu S3,4, Lee JH3,5, Choi SH3,4, Kim H1,2,3.

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Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Interdisciplinary Program in Bioinformatics, Seoul National University, Seoul, Republic of Korea.
Food-Borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul, Republic of Korea.
Department of Agricultural Biotechnology, Center for Food Safety and Toxicology, Seoul National University, Seoul, Republic of Korea.
Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University, Yongin, Republic of Korea.



Bacillus cereus is well known as a gastrointestinal pathogen that causes food-borne illness. In the present study, we sequenced the complete genome of B. cereus FORC_013 isolated from fried eel in South Korea. To extend our understanding of the genomic characteristics of FORC_013, we conducted a comparative analysis with the published genomes of other B. cereus strains.


We fully assembled the single circular chromosome (5,418,913 bp) and one plasmid (259,749 bp); 5511 open reading frames (ORFs) and 283 ORFs were predicted for the chromosome and plasmid, respectively. Moreover, we detected that the enterotoxin (NHE, HBL, CytK) induces food-borne illness with diarrheal symptom, and that the pleiotropic regulator, along with other virulence factors, plays a role in surviving and biofilm formation. Through comparative analysis using the complete genome sequence of B. cereus FORC_013, we identified both positively selected genes related to virulence regulation and 224 strain-specific genes of FORC_013.


Through genome analysis of B. cereus FORC_013, we identified multiple virulence factors that may contribute to pathogenicity. These results will provide insight into further studies regarding B. cereus pathogenesis mechanism at the genomic level.


Bacillus cereus; Comparative genomics; Positive selection; Unique genes; Virulence

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