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Cell Rep. 2017 May 16;19(7):1431-1443. doi: 10.1016/j.celrep.2017.04.068.

A Murine Intestinal Intraepithelial NKp46-Negative Innate Lymphoid Cell Population Characterized by Group 1 Properties.

Author information

1
Laboratory of Experimental Immunology, Ghent University, 9000 Ghent, Belgium; Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, 171 77 Stockholm, Sweden.
2
Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg University Mainz, 55131 Mainz, Germany; Max-Planck-Institute of Immunobiology and Epigenetics, 79108 Freiburg, Germany.
3
Institute of Inflammation and Neurodegeneration, Otto-von-Guericke University Magdeburg, 39120 Magdeburg, Germany.
4
VIB Inflammation Research Centre, 9000 Ghent, Belgium.
5
Laboratory of Experimental Immunology, Ghent University, 9000 Ghent, Belgium.
6
Laboratory of Immunobiology, Rega Institute for Medical Research, Department of Microbiology and Immunology, KU Leuven - University of Leuven, 3000 Leuven, Belgium.
7
Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg University Mainz, 55131 Mainz, Germany; Department of Microbiology, Charité - University Medical Centre Berlin, 12203 Berlin, Germany.
8
Laboratory of Experimental Immunology, Ghent University, 9000 Ghent, Belgium. Electronic address: georges.leclercq@ugent.be.

Abstract

The Ly49E receptor is preferentially expressed on murine innate-like lymphocytes, such as epidermal Vγ3 T cells, intestinal intraepithelial CD8αα+ T lymphocytes, and CD49a+ liver natural killer (NK) cells. As the latter have recently been shown to be distinct from conventional NK cells and have innate lymphoid cell type 1 (ILC1) properties, we investigated Ly49E expression on intestinal ILC populations. Here, we show that Ly49E expression is very low on known ILC populations, but it can be used to define a previously unrecognized intraepithelial innate lymphoid population. This Ly49E-positive population is negative for NKp46 and CD8αα, expresses CD49a and CD103, and requires T-bet expression and IL-15 signaling for differentiation and/or survival. Transcriptome analysis reveals a group 1 ILC gene profile, different from NK cells, iCD8α cells, and intraepithelial ILC1. Importantly, NKp46-CD8αα-Ly49E+ cells produce interferon (IFN)-γ, suggesting that this previously unrecognized population may contribute to Th1-mediated immunity.

KEYWORDS:

IFN-γ; ILC1; Ly49E; NKp46-negative; intestinal; intraepithelial

PMID:
28514662
DOI:
10.1016/j.celrep.2017.04.068
[Indexed for MEDLINE]
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