Format

Send to

Choose Destination
J Nanobiotechnology. 2017 May 16;15(1):39. doi: 10.1186/s12951-017-0275-4.

Polymeric micelles with dual thermal and reactive oxygen species (ROS)-responsiveness for inflammatory cancer cell delivery.

Author information

1
School of Pharmaceutical Sciences, Chongqing University, 55 South Daxuecheng Road, Chongqing, 401331, China.
2
School of Pharmaceutical Sciences, Chongqing University, 55 South Daxuecheng Road, Chongqing, 401331, China. ping.hu@cqu.edu.cn.
3
NorthWest Centre of Advanced Drug Delivery (NoWCADD), School of Pharmacy, and Centre for Tissue Injury and Repair, Institute of Inflammation and Repair, University of Manchester, Oxford Road, Manchester, M13 9PT, UK.
4
First Affiliated Hospital of the Medical College, Shihezi University, Xinjiang, 832008, People's Republic of China.
5
School of Pharmaceutical Sciences, Chongqing University, 55 South Daxuecheng Road, Chongqing, 401331, China. yun.he@cqu.edu.cn.

Abstract

BACKGROUND:

The object of this study was to develop a thermally and reactive oxygen species-responsive nanocarrier system for cancer therapy.

RESULTS:

PPS-PNIPAm block copolymer was designed and synthesised using a combination of living anionic ring-opening polymerization and atom transfer radical polymerization. The synthesized polymer formed micellar aggregates in water and demonstrated dual responsiveness towards temperature and oxidants. Using doxorubicin (DOX) as a model drug, encapsulation and in vitro release of the drug molecules in PPS-PNIPAm nanocarriers confirmed the responsive release properties of such system. Cell uptake of the DOX loaded micelles was investigated with human breast cancer cell line (MCF-7). The results showed Dox-loaded micelles were able to be taken by the cells and mainly reside in the cytoplasma. In the stimulated cells with an elevated level of ROS, more released DOX was observed around the nuclei. In the cytotoxicity experiments, the Dox-loaded micelles demonstrated comparable efficacy to free DOX at higher concentrations, especially on ROS stimulated cells.

CONCLUSIONS:

These results demonstrated that PPS-PNIPAm nanocarriers possess the capability to respond two typical stimuli in inflammatory cells: temperature and oxidants and can be used in anticancer drug delivery.

KEYWORDS:

Cellular uptake; Nanomedicine; Polymeric micelle; ROS-responsive

PMID:
28511687
PMCID:
PMC5434630
DOI:
10.1186/s12951-017-0275-4
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center