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Med Res Rev. 2018 Mar;38(2):504-524. doi: 10.1002/med.21444. Epub 2017 May 16.

Application of Combination High-Throughput Phenotypic Screening and Target Identification Methods for the Discovery of Natural Product-Based Combination Drugs.

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Department of Biology, Georgia State University, Atlanta, GA, 30303.
Incan Solutions Private Limited, Nova Scotia, Canada.
Novazoi Theranostics, Rolling Hills Estates, CA, 90274.


Modern drug discovery efforts have had mediocre success rates with increasing developmental costs, and this has encouraged pharmaceutical scientists to seek innovative approaches. Recently with the rise of the fields of systems biology and metabolomics, network pharmacology (NP) has begun to emerge as a new paradigm in drug discovery, with a focus on multiple targets and drug combinations for treating disease. Studies on the benefits of drug combinations lay the groundwork for a renewed focus on natural products in drug discovery. Natural products consist of a multitude of constituents that can act on a variety of targets in the body to induce pharmacodynamic responses that may together culminate in an additive or synergistic therapeutic effect. Although natural products cannot be patented, they can be used as starting points in the discovery of potent combination therapeutics. The optimal mix of bioactive ingredients in natural products can be determined via phenotypic screening. The targets and molecular mechanisms of action of these active ingredients can then be determined using chemical proteomics, and by implementing a reverse pharmacokinetics approach. This review article provides evidence supporting the potential benefits of natural product-based combination drugs, and summarizes drug discovery methods that can be applied to this class of drugs.


chemical proteomics; combination drugs; multicomponent drugs; network pharmacology; phenotypic screening; reverse pharmacokinetics

[Indexed for MEDLINE]

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