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Nat Commun. 2017 May 16;8:15261. doi: 10.1038/ncomms15261.

A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice.

Laronda MM1,2,3, Rutz AL4,5, Xiao S1,2,3, Whelan KA1,2,3, Duncan FE1,2,3,6, Roth EW7, Woodruff TK1,2,3, Shah RN4,5,8,9.

Author information

1
Division of Reproductive Biology in Medicine, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.
2
Center for Reproductive Science, Northwestern University, Chicago, Illinois 60611, USA.
3
Oncofertility Consortium, Northwestern University, Chicago, Illinois 60611, USA.
4
Simpson Querrey Institute for BioNanotechnology, Northwestern University, Chicago, Illinois 60611, USA.
5
Department of Biomedical Engineering, Northwestern University, Evanston, Illinois 60208, USA.
6
Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA.
7
Northwestern University Atomic and Nanoscale Characterization Experimental Center, Northwestern University, Evanston, Illinois 60208, USA.
8
Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois 60208, USA.
9
Department of Surgery, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA.

Abstract

Emerging additive manufacturing techniques enable investigation of the effects of pore geometry on cell behavior and function. Here, we 3D print microporous hydrogel scaffolds to test how varying pore geometry, accomplished by manipulating the advancing angle between printed layers, affects the survival of ovarian follicles. 30° and 60° scaffolds provide corners that surround follicles on multiple sides while 90° scaffolds have an open porosity that limits follicle-scaffold interaction. As the amount of scaffold interaction increases, follicle spreading is limited and survival increases. Follicle-seeded scaffolds become highly vascularized and ovarian function is fully restored when implanted in surgically sterilized mice. Moreover, pups are born through natural mating and thrive through maternal lactation. These findings present an in vivo functional ovarian implant designed with 3D printing, and indicate that scaffold pore architecture is a critical variable in additively manufactured scaffold design for functional tissue engineering.

PMID:
28509899
PMCID:
PMC5440811
DOI:
10.1038/ncomms15261
[Indexed for MEDLINE]
Free PMC Article

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