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Plant Reprod. 2017 Jun;30(2):81-94. doi: 10.1007/s00497-017-0301-6. Epub 2017 May 16.

Untargeted metabolomic analysis of tomato pollen development and heat stress response.

Author information

1
Plant Breeding, Wageningen University and Research Centre, PO Box 386, 6700 AJ, Wageningen, The Netherlands.
2
Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.
3
Plant Breeding, Wageningen University and Research Centre, PO Box 386, 6700 AJ, Wageningen, The Netherlands. arnaud.bovy@wur.nl.

Abstract

Pollen development metabolomics. Developing pollen is among the plant structures most sensitive to high temperatures, and a decrease in pollen viability is often associated with an alteration of metabolite content. Most of the metabolic studies of pollen have focused on a specific group of compounds, which limits the identification of physiologically important metabolites. To get a better insight into pollen development and the pollen heat stress response, we used a liquid chromatography-mass spectrometry platform to detect secondary metabolites in pollen of tomato (Solanum lycopersicum L.) at three developmental stages under control conditions and after a short heat stress at 38 °C. Under control conditions, the young microspores accumulated a large amount of alkaloids and polyamines, whereas the mature pollen strongly accumulated flavonoids. The heat stress treatment led to accumulation of flavonoids in the microspore. The biological role of the detected metabolites is discussed. This study provides the first untargeted metabolomic analysis of developing pollen under a changing environment that can serve as reference for further studies.

KEYWORDS:

Heat stress; High temperature; Metabolomics; Pollen development; Untargeted analysis

PMID:
28508929
PMCID:
PMC5486769
DOI:
10.1007/s00497-017-0301-6
[Indexed for MEDLINE]
Free PMC Article

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