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Elife. 2017 May 16;6. pii: e23235. doi: 10.7554/eLife.23235.

Voltage-gated Na+ currents in human dorsal root ganglion neurons.

Author information

1
Department of Urology, The Second Hospital of Shandong University, Jinan Shi, China.
2
Lilly Research Laboratories, Indianapolis, United States.
3
Office of Research on Women's Health, National Institutes of Health, Bethesda, United States.
4
Department of Neurobiology, University of Pittsburgh School of Medicine, Pittsburgh, United States.

Abstract

Available evidence indicates voltage-gated Na+ channels (VGSCs) in peripheral sensory neurons are essential for the pain and hypersensitivity associated with tissue injury. However, our understanding of the biophysical and pharmacological properties of the channels in sensory neurons is largely based on the study of heterologous systems or rodent tissue, despite evidence that both expression systems and species differences influence these properties. Therefore, we sought to determine the extent to which the biophysical and pharmacological properties of VGSCs were comparable in rat and human sensory neurons. Whole cell patch clamp techniques were used to study Na+ currents in acutely dissociated neurons from human and rat. Our results indicate that while the two major current types, generally referred to as tetrodotoxin (TTX)-sensitive and TTX-resistant were qualitatively similar in neurons from rats and humans, there were several differences that have important implications for drug development as well as our understanding of pain mechanisms.

KEYWORDS:

NaV1.7; NaV1.8; cell culture; human; local anesthetics; neuroscience

PMID:
28508747
PMCID:
PMC5433841
DOI:
10.7554/eLife.23235
[Indexed for MEDLINE]
Free PMC Article

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