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Leuk Lymphoma. 2017 Dec;58(12):2939-2942. doi: 10.1080/10428194.2017.1317095. Epub 2017 May 16.

High frequency of identical clonal immunoglobulin DNA in pre-treatment tumor and plasma from untreated patients with HIV-associated lymphoma: prospective multicenter trial of the AIDS malignancies consortium (AMC 064).

Author information

1
a Sidney Kimmel Comprehensive Cancer Center Johns Hopkins University , Baltimore , MD , USA.
2
b University of Arkansas for Medical Sciences , Little Rock , AK , USA.
3
c Memorial Sloan Kettering Cancer Center , New York , NY , USA.
4
d Siteman Cancer Center, Washington University , St. Louis , MO , USA.
5
e Pennsylvania Oncology , Philadelphia , PA , USA.
6
f George Washington University , Washington D.C , USA.
7
g Adaptive Biotechnologies, Corp. , South San Francisco , CA , USA.

Abstract

Patients with HIV are at increased risk for developing B-cell lymphomas likely due in part to chronic antigen stimulation leading to clonal immunoglobulin (Ig) gene rearrangements. Next-generation sequencing (NGS)-based identification of circulating Ig clonotypes has not been well-characterized in HIV-related lymphomas. The AIDS Malignancies Consortium (AMC) enrolled 51 untreated patients with HIV-related B-cell lymphomas and analyzed paired tumor/plasma specimens for Ig clonotypes using an NGS approach (AMC064, NCT00981097). Lymphoma-specific clonotypes (>5% frequency) were identified in 83% (33/40) of tumor specimens. Results from paired tumor/plasma specimens showed identical circulating clonotypes in the plasma from 97% (32/33) of patients. High International Prognostic Index (IPI) scores of 3-4 among patients with B-cell lymphoma correlated with higher lymphoma molecules/million diploid genomes in the plasma compared with lower IPI scores of 0-2, median 77335 vs. 6876, pā€‰=ā€‰.005. Further studies are merited to determine whether plasma clonal Ig DNA is prognostic in HIV-related lymphomas.

KEYWORDS:

DNA; HIV; Immunoglobulin; Lymphoma; clones

PMID:
28508728
PMCID:
PMC5867902
DOI:
10.1080/10428194.2017.1317095
[Indexed for MEDLINE]
Free PMC Article

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