Format

Send to

Choose Destination
Proc Natl Acad Sci U S A. 2017 May 30;114(22):E4511-E4519. doi: 10.1073/pnas.1705233114. Epub 2017 May 15.

Critical roles of DNA demethylation in the activation of ripening-induced genes and inhibition of ripening-repressed genes in tomato fruit.

Author information

1
Shanghai Center for Plant Stress Biology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China; jkzhu@purdue.edu zblang@sibs.ac.cn.
2
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907.
3
Shanghai Institute of Plant Physiology and Ecology and Center of Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

Abstract

DNA methylation is a conserved epigenetic mark important for genome integrity, development, and environmental responses in plants and mammals. Active DNA demethylation in plants is initiated by a family of 5-mC DNA glycosylases/lyases (i.e., DNA demethylases). Recent reports suggested a role of active DNA demethylation in fruit ripening in tomato. In this study, we generated loss-of-function mutant alleles of a tomato gene, SlDML2, which is a close homolog of the Arabidopsis DNA demethylase gene ROS1 In the fruits of the tomato mutants, increased DNA methylation was found in thousands of genes. These genes included not only hundreds of ripening-induced genes but also many ripening-repressed genes. Our results show that SlDML2 is critical for tomato fruit ripening and suggest that active DNA demethylation is required for both the activation of ripening-induced genes and the inhibition of ripening-repressed genes.

KEYWORDS:

5-mC DNA glycosylase; DNA demethylase; DNA methylation; epigenetic regulation; gene silencing

PMID:
28507144
PMCID:
PMC5465898
DOI:
10.1073/pnas.1705233114
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center