Colistin Resistance in Carbapenem-Resistant Klebsiella pneumoniae Mediated by Chromosomal Integration of Plasmid DNA

Antimicrob Agents Chemother. 2017 Jul 25;61(8):e00404-17. doi: 10.1128/AAC.00404-17. Print 2017 Aug.

Abstract

Here we describe the spread of colistin resistance in clinical isolates of carbapenem-resistant Klebsiella pneumoniae in Medellín, Colombia. Among 32 isolates collected between 2012 and 2014, 24 showed genetic alterations in mgrB Nineteen isolates belonged to sequence type 512 (ST512) (or its single locus variant [SLV]) and harbored an 8.1-kb hsdMSR insertion corresponding to ISKpn25, indicating a clonal expansion of the resistant strain. The insertion region showed 100% identity to several plasmids, suggesting that the colistin resistance is mediated by chromosomal integration of plasmid DNA.

Keywords: Klebsiella pneumoniae; carbapenem resistance; colistin resistance; mgrB.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Carbapenem-Resistant Enterobacteriaceae / genetics
  • Carbapenems / pharmacology
  • Colistin / pharmacology*
  • Drug Resistance, Bacterial / genetics*
  • Humans
  • Klebsiella pneumoniae / drug effects*
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Membrane Proteins / genetics
  • Mutagenesis, Insertional / genetics
  • Plasmids / genetics*

Substances

  • Anti-Bacterial Agents
  • Carbapenems
  • Membrane Proteins
  • Colistin