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BMC Genet. 2017 May 15;18(1):41. doi: 10.1186/s12863-017-0506-6.

Sucrose non-ferment 1 related protein kinase 2 (SnRK2) genes could mediate the stress responses in potato (Solanum tuberosum L.).

Bai J1,2, Mao J3,4, Yang H3,4, Khan A5, Fan A6, Liu S6, Zhang J3,4, Wang D3,6, Gao H7, Zhang J8,9.

Author information

1
Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou, 730070, Gansu, People's Republic of China. baijp@gsau.edu.cn.
2
College of Agronomy, Gansu Agricultural University, Lanzhou, 730070, Gansu, People's Republic of China. baijp@gsau.edu.cn.
3
Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou, 730070, Gansu, People's Republic of China.
4
College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, Gansu, People's Republic of China.
5
International Potato Center (CIP), Avenida La Molina 1895, La Molina Apartado, 1558, Lima, Peru.
6
College of Agronomy, Gansu Agricultural University, Lanzhou, 730070, Gansu, People's Republic of China.
7
State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, Gansu, People's Republic of China.
8
Gansu Key Lab of Crop Improvement & Germplasm Enhancement, Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou, 730070, Gansu, People's Republic of China. jlzhang@lzu.edu.cn.
9
State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, Gansu, People's Republic of China. jlzhang@lzu.edu.cn.

Abstract

BACKGROUND:

The SnRKs (sucrose non-fermenting 1 related protein kinase) are a gene family coding for Ser/Thr protein kinases and play important roles in linking the tolerance and metabolic responses of plants to abiotic stresses. To date, no genome-wide characterization of the sucrose non-ferment 1 related protein kinase 2 (SnRK2) subfamily has been conducted in potato (Solanum tuberosum L.).

RESULTS:

In this study, eight StSnRK2 genes (StSnRK2.1- StSnRK2.8) were identified in the genome of the potato (Solanum tuberosum L.) cultivar 'Longshu 3', with similar characteristics to SnRK2 from other plant species in gene structure, motif distribution and secondary structures. The C-terminal regions were highly divergent among StSnRK2s, while they all carried the similar Ser/Thr protein kinase domain. The fluorescence of GFP fused with StSnRK2.1, StSnRK2.2, StSnRK2.6, StSnRK2.7 and StSnRK2.8 was detected in the nucleus and cytoplasm of onion epidermal cells with StSnRK2.3 and StSnRK2.4 mainly associated to the nucleus while StSnRK2.5 to subcellular organelles. Expression level analysis by qRT-PCR showed that StSnRK2.1, 2.2, 2.5 and 2.6 were more than 1 fold higher in the root than in the leaf, tuber and stem tissues. The expressions of StSnRK2.3, 2.7, and 2.8 were at least 1.5 folds higher in the leaf and stem than in the root, but lower in the tuber. The expression of StSnRK2.4 was also significantly (P < 0.05) higher in leaf, stem, and tuber than in the root. From the perspective of the relative expressions of StSnRK2 genes in potato, ABA treatment had a different effect from NaCl and PEG treatments.

CONCLUSION:

In the present study, we identified and characterized eight SnRK2s in the potato genome. The eight StSnRK2s exhibit similar gene structure and secondary structures in potato to the SnRK2s found in other plant species. The relative expression of eight genes varied among various tissues (roots, leaves, tubers, and stems) and abiotic stresses (ABA, NaCl and PEG-6000) with the prolongation of treatments. This study provides valuable information for the future functional dissection of potato SnRK2 genes in stress signal transduction, plant growth and development.

KEYWORDS:

ABA; NaCl; PEG-6000; Potato; StSnRK2; Stress responses

PMID:
28506210
PMCID:
PMC5433004
DOI:
10.1186/s12863-017-0506-6
[Indexed for MEDLINE]
Free PMC Article

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