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Nat Methods. 2017 Jul;14(7):695-698. doi: 10.1038/nmeth.4294. Epub 2017 May 15.

Nm-seq maps 2'-O-methylation sites in human mRNA with base precision.

Author information

1
Department of Chemistry, The University of Chicago, Chicago, Illinois, USA.
2
Howard Hughes Medical Institute, The University of Chicago, Chicago, Illinois, USA.
3
Cancer Research Center, Chaim Sheba Medical Center, Tel Hashomer, Israel.
4
Wohl Centre for Translational Medicine, Chaim Sheba Medical Center, Tel-Hashomer, Israel.
5
The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
6
Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
7
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
8
Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA.

Abstract

The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, the inert chemical nature of Nm still limits sensitivity and precludes mapping in mRNA. We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. Nm-seq uncovered thousands of Nm sites in human mRNA with features suggesting functional roles.

PMID:
28504680
PMCID:
PMC5712428
DOI:
10.1038/nmeth.4294
[Indexed for MEDLINE]
Free PMC Article

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