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New Phytol. 2017 Jul;215(1):157-172. doi: 10.1111/nph.14586. Epub 2017 May 15.

Identification of factors required for m6 A mRNA methylation in Arabidopsis reveals a role for the conserved E3 ubiquitin ligase HAKAI.

Author information

1
Functional Genomics and Proteomics of Plants, Central European Institute of Technology and National Centre for Biomolecular Research, Masaryk University, 62500, Brno, Czech Republic.
2
Institute of Biotechnology, University of Helsinki, 00014, Helsinki, Finland.
3
Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE12 5RD, UK.
4
Research Center in Biodiversity and Genetic Resources, University of Porto, 4485-661 Vairão, Portugal.
5
Department of Plant Systems Biology, VIB, 9052, Gent, Belgium.
6
Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052, Gent, Belgium.
7
Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan, Shanxi, 030031, China.
8
The State Key Laboratory of Agrobiotechnology, The School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China.
9
School of Veterinary Medicine and Sciences, University of Nottingham, Sutton Bonington, Loughborough, LE12 5RD, UK.
10
Sainsbury Laboratory, University of Cambridge, Cambridge, CB2 1LR, UK.

Abstract

N6-adenosine methylation (m6 A) of mRNA is an essential process in most eukaryotes, but its role and the status of factors accompanying this modification are still poorly understood. Using combined methods of genetics, proteomics and RNA biochemistry, we identified a core set of mRNA m6 A writer proteins in Arabidopsis thaliana. The components required for m6 A in Arabidopsis included MTA, MTB, FIP37, VIRILIZER and the E3 ubiquitin ligase HAKAI. Downregulation of these proteins led to reduced relative m6 A levels and shared pleiotropic phenotypes, which included aberrant vascular formation in the root, indicating that correct m6 A methylation plays a role in developmental decisions during pattern formation. The conservation of these proteins amongst eukaryotes and the demonstration of a role in writing m6 A for the E3 ubiquitin ligase HAKAI is likely to be of considerable relevance beyond the plant sciences.

KEYWORDS:

Arabidopsis ; HAKAI; N6-adenosine methylation (m6A); VIRILIZER; mRNA methylation; protoxylem

PMID:
28503769
PMCID:
PMC5488176
DOI:
10.1111/nph.14586
[Indexed for MEDLINE]
Free PMC Article

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