Format

Send to

Choose Destination
Fish Shellfish Immunol. 2017 Jul;66:207-216. doi: 10.1016/j.fsi.2017.05.029. Epub 2017 May 10.

Local and systemic humoral immune response in farmed Atlantic salmon (Salmo salar L.) under a natural amoebic gill disease outbreak.

Author information

1
Marine and Freshwater Research Centre, Galway-Mayo Institute of Technology, Dublin Road, Galway, Co. Galway, Ireland; FishVet Group Ireland, Unit 7b Oranmore Business Park, Oranmore, Co. Galway, Ireland. Electronic address: mar.marcos.lopez@gmail.com.
2
Department of Cell Biology and Histology, Faculty of Biology, Campus Regional de Excelencia Internacional "Campus Mare Nostrum", University of Murcia, Murcia, Spain.
3
FishVet Group Ireland, Unit 7b Oranmore Business Park, Oranmore, Co. Galway, Ireland.
4
Marine and Freshwater Research Centre, Galway-Mayo Institute of Technology, Dublin Road, Galway, Co. Galway, Ireland.

Abstract

Amoebic gill disease (AGD), caused by the protozoan parasite Neoparamoeba perurans, is one of the most significant infectious diseases for Atlantic salmon (Salmo salar L.) mariculture. The present study investigated the humoral immune response (both local in gill mucus and systemic in serum) of farmed Atlantic salmon naturally infected with N. perurans in commercial sea pens, at two different stages of the disease and after freshwater treatment. Parameters analysed included activity of immune related enzymes (i.e. lysozyme, peroxidase, protease, anti-protease, esterase, alkaline phosphatase), IgM levels, and the terminal carbohydrate profile in the gill mucus. Overall, greater variations between groups were noted in the immune parameters determined in gill mucus than the equivalent in the serum. In gill mucus, IgM levels and peroxidase, lysozyme, esterase and protease activities were decreased in fish showing longer exposure time to the infection and higher disease severity, then showed a sequential increase after treatment. Results obtained highlight the capacity of gills to elicit a local response to the infection, indicate an impaired immune response at the later stages of the disease, and show partial reestablishment of the host immune status after freshwater treatment. In addition to providing data on the humoral response to AGD, this study increases knowledge on gill mucosal humoral immunity, since some of the parameters were analysed for the first time in gill mucus.

KEYWORDS:

Amoebic gill disease; Atlantic salmon (Salmo salar L.); Gill mucus; Humoral immunity; Serum

PMID:
28501445
DOI:
10.1016/j.fsi.2017.05.029
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center