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EMBO Rep. 2017 Jul;18(7):1231-1247. doi: 10.15252/embr.201744059. Epub 2017 May 12.

Transposon-driven transcription is a conserved feature of vertebrate spermatogenesis and transcript evolution.

Author information

1
European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Cambridge, UK.
2
European Molecular Biology Laboratory, Mouse Biology Outstation, Monterotondo, Italy.
3
MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
4
Institut Pasteur - Bioinformatics and Biostatistics Hub, C3BI, USR 3756 IP CNRS, Paris, France.
5
Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
6
Institut Curie - CNRS UMR3215, INSERM U934, Paris, France.
7
Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany.
8
European Molecular Biology Laboratory, Mouse Biology Outstation, Monterotondo, Italy donal.ocarroll@ed.ac.uk aje@ebi.ac.uk.
9
European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Cambridge, UK donal.ocarroll@ed.ac.uk aje@ebi.ac.uk.

Abstract

Spermatogenesis is associated with major and unique changes to chromosomes and chromatin. Here, we sought to understand the impact of these changes on spermatogenic transcriptomes. We show that long terminal repeats (LTRs) of specific mouse endogenous retroviruses (ERVs) drive the expression of many long non-coding transcripts (lncRNA). This process occurs post-mitotically predominantly in spermatocytes and round spermatids. We demonstrate that this transposon-driven lncRNA expression is a conserved feature of vertebrate spermatogenesis. We propose that transposon promoters are a mechanism by which the genome can explore novel transcriptional substrates, increasing evolutionary plasticity and allowing for the genesis of novel coding and non-coding genes. Accordingly, we show that a small fraction of these novel ERV-driven transcripts encode short open reading frames that produce detectable peptides. Finally, we find that distinct ERV elements from the same subfamilies act as differentially activated promoters in a tissue-specific context. In summary, we demonstrate that LTRs can act as tissue-specific promoters and contribute to post-mitotic spermatogenic transcriptome diversity.

KEYWORDS:

endogenous retroviruses; genome evolution; lncRNA; spermatogenesis; transcriptome

PMID:
28500258
PMCID:
PMC5494522
DOI:
10.15252/embr.201744059
[Indexed for MEDLINE]
Free PMC Article

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