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J Cell Biol. 2017 Jun 5;216(6):1597-1608. doi: 10.1083/jcb.201604079. Epub 2017 May 12.

The chromokinesin Klp3a and microtubules facilitate acentric chromosome segregation.

Author information

1
Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064.
2
Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94143.
3
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94143.
4
Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064 wtsulliv@ucsc.edu.

Abstract

Although poleward segregation of acentric chromosomes is well documented, the underlying mechanisms remain poorly understood. Here, we demonstrate that microtubules play a key role in poleward movement of acentric chromosome fragments generated in Drosophila melanogaster neuroblasts. Acentrics segregate with either telomeres leading or lagging in equal frequency and are preferentially associated with peripheral bundled microtubules. In addition, laser ablation studies demonstrate that segregating acentrics are mechanically associated with microtubules. Finally, we show that successful acentric segregation requires the chromokinesin Klp3a. Reduced Klp3a function results in disorganized interpolar microtubules and shortened spindles. Normally, acentric poleward segregation occurs at the periphery of the spindle in association with interpolar microtubules. In klp3a mutants, acentrics fail to localize and segregate along the peripheral interpolar microtubules and are abnormally positioned in the spindle interior. These studies demonstrate an unsuspected role for interpolar microtubules in driving acentric segregation.

PMID:
28500183
PMCID:
PMC5461011
DOI:
10.1083/jcb.201604079
[Indexed for MEDLINE]
Free PMC Article

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