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Stem Cell Res. 2017 May;21:148-159. doi: 10.1016/j.scr.2017.04.009. Epub 2017 Apr 21.

Initial clonogenic potential of human endothelial progenitor cells is predictive of their further properties and establishes a functional hierarchy related to immaturity.

Author information

1
INSERM U1197, Hôpital Paul Brousse, Villejuif, France.
2
ERL CNRS 9215, Laboratoire CRRET, Université Paris Est Créteil, Faculté des Sciences et Technologies, Créteil, France.
3
Center for Interdisciplinary Research in Biology, Collège de France, Paris, France; Inserm U1050, Paris, France; CNRS UMRS 7241, Paris, France.
4
INSERM U1197, Hôpital Paul Brousse, Villejuif, France. Electronic address: georges.uzan@inserm.fr.

Abstract

Endothelial progenitor cells (EPCs) generate in vitro Endothelial Colony Forming Cells (ECFCs) combining features of endothelial and stem/progenitor cells. Their angiogenic properties confer them a therapeutic potential for treating ischemic lesions. They may be isolated from umbilical cord blood (CB-ECFCs) or peripheral adult blood (AB-ECFCs). It is generally accepted that CB-ECFCs are more clonogenic, proliferative and angiogenic than AB-ECFCs. Nevertheless, only a few studies have focused on the functional heterogeneity of CB-ECFCs from different individuals. Moreover, AB-ECFC loss of function is yet to be precisely described. We have focused on these two issues that are critical for clinical perspectives. The detailed clonogenic profile of CB-ECFCs and AB-ECFCs was obtained and revealed a high inter individual heterogeneity and the absence of correlation with age. Most CB-ECFCs yielded initial colonies and had functional properties similar to those of AB-ECFCs. Conversely, a high clonogenicity was associated with an enhanced proliferative and angiogenic potential and stemness gene overexpression, confirming that immaturity, lost by AB-ECFCs, was a prerequisite to functionality. We thus demonstrated the importance of selecting CB-ECFCs according to specific criteria, and we propose using the initial clonogenicity as a relevant marker of their potential efficacy on vascular repair.

KEYWORDS:

Cord blood; Endothelial Colony Forming Cells; Endothelial progenitor cells; Functional hierarchy; Immaturity; Peripheral adult blood; Senescence

PMID:
28499264
DOI:
10.1016/j.scr.2017.04.009
[Indexed for MEDLINE]
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