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Nutrients. 2017 May 12;9(5). pii: E490. doi: 10.3390/nu9050490.

Desalted Duck Egg White Peptides Promote Calcium Uptake and Modulate Bone Formation in the Retinoic Acid-Induced Bone Loss Rat and Caco-2 Cell Model.

Hou T1,2, Liu Y3,4, Kolba N5, Guo D6,7, He H8,9.

Author information

1
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. mrhoutao@webmail.hzau.edu.cn.
2
Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. mrhoutao@webmail.hzau.edu.cn.
3
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. ysliu@webmail.hzau.edu.cn.
4
Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. ysliu@webmail.hzau.edu.cn.
5
USDA-ARS, Robert W. Holley Center for Agriculture and Health, Cornell University, Ithaca, NY 14850, USA. nk598@cornell.edu.
6
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. guodanj@webmail.hzau.edu.cn.
7
Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. guodanj@webmail.hzau.edu.cn.
8
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. hehui@mail.hzau.edu.cn.
9
Key Laboratory of Environment Correlative Dietology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China. hehui@mail.hzau.edu.cn.

Abstract

Desalted duck egg white peptides (DPs) have been proven to promote calcium uptake in Caco-2 cells and rats treated with a calcium-deficient diet. The retinoic acid-induced bone loss model was used to evaluate the effect of DPs on calcium absorption and bone formation. Three-month-old Wistar female rats were treated with 0.9% saline, DPs (800 mg/kg), or alendronate (5 mg/kg) for three weeks immediately after retinoic acid treatment (80 mg/kg) once daily for two weeks. The model group was significantly higher in serum bone alkaline phosphatase than the other three groups (p < 0.05), but lower in calcium absorption rate, serum osteocalcin, bone weight index, bone calcium content, bone mineral density, and bone max load. After treatment with DPs or alendronate, the absorption rate increased and some serum and bone indices recovered. The morphology results indicated bone tissue form were ameliorated and numbers of osteoclasts decreased after supplementation with DPs or alendronate. The in vitro study showed that the transient receptor potential vanilloid 6 (TRPV6) calcium channel was the main transport pathway of both DPs and Val-Ser-Glu-Glu peptitde (VSEE), which was identified from DPs. Our results indicated that DPs could be a promising alternative to current therapeutic agents for bone loss because of the promotion of calcium uptake and regulation of bone formation.

KEYWORDS:

alendronate; desalted duck egg white peptides (DPs); retinoic acid; transient receptor potential vanilloid 6 (TRPV6) calcium channel

PMID:
28498349
DOI:
10.3390/nu9050490
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