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Elife. 2017 May 12;6. pii: e24630. doi: 10.7554/eLife.24630.

A selectivity filter at the intracellular end of the acid-sensing ion channel pore.

Author information

1
Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
2
School of Science, RMIT University, Melbourne, Australia.

Abstract

Increased extracellular proton concentrations during neurotransmission are converted to excitatory sodium influx by acid-sensing ion channels (ASICs). 10-fold sodium/potassium selectivity in ASICs has long been attributed to a central constriction in the channel pore, but experimental verification is lacking due to the sensitivity of this structure to conventional manipulations. Here, we explored the basis for ion selectivity by incorporating unnatural amino acids into the channel, engineering channel stoichiometry and performing free energy simulations. We observed no preference for sodium at the "GAS belt" in the central constriction. Instead, we identified a band of glutamate and aspartate side chains at the lower end of the pore that enables preferential sodium conduction.

KEYWORDS:

ACIC; ENaC/DEG; biophysics; ion permeation; molecular dynamics; neuroscience; sodium selectivity; structural biology; unnatural amino acids; xenopus

PMID:
28498103
PMCID:
PMC5449180
DOI:
10.7554/eLife.24630
[Indexed for MEDLINE]
Free PMC Article

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